We recently reported that iPSCs can

2018-10-20

We recently reported that iPSCs can be generated using non-modified mRNA (Arnold et al., 2012). In this report we will focus on the crucial steps for successfully applying this method. Material and methods Results Discussion Acknowledgments The work was supported by funding from the Ger

CBP is implicated in different biological functions

2018-10-20

CBP is implicated in different biological functions with an essential role in maintaining normal germ cells development. CBP is expressed in primordial germ cells (PGCs) and in ESCs but is highly expressed in PGCs compared with ESCs. Proper levels of CBP have been shown to be important for hematopoi

Dental pulp stem cells DPSCs also known as dental

2018-10-20

Dental pulp stem EZ Cap Reagent GG Supplier (DPSCs), also known as dental pulp-derived mesenchymal stem cells, are a multipotent adult stem cell population that has a high proliferative potential. DPSCs in this study isolated from adult teeth are easily accessible and cryopreservable for long perio

Muscle resident Sca CD cells

2018-10-20

Muscle-resident Sca-1+CD45− flunixin meglumine were minimally responsive to HFD and/or endurance exercise. A significant reduction in VEGF gene expression was observed in MDSCs in response to HFD, yet no other changes were noted in MDSC relative quantity or the expression of genes related to ECM re

br Introduction A key question

2018-10-20

Introduction A key question in cardiovascular biology is to what degree the heart is able to regenerate after tissue damage from either cardiac stem cells or cardiomyocyte division. Cardiovascular disease including myocardial infarct is currently one of the leading causes of death worldwide, and

br Introduction Mammary gland development is controlled

2018-10-20

Introduction Mammary gland development is controlled by the endocrine system, in particular by the ovarian steroid hormones, estrogen and progesterone, and by the pituitary hormones, growth hormone (GH) and prolactin. Studies in animal models showed that GH deficiency impairs mammary gland develo

In D the generation of confluent monolayers ahead

2018-10-20

In 2D, the generation of confluent monolayers ahead of differentiation was noted to be critical for efficient cardiomyogenesis (Lian et al., 2013). This suggests that the cell density, which impacts on cell-cell contacts and the concentration of paracrine factors, plays a role. Lian et al. (2013) th

Introduction Cumulative lifetime hormone exposure as a resul

2018-10-20

Introduction Cumulative lifetime hormone exposure as a result of natural menstrual cycles and exogenous hormone therapies is a strong determinant of increased breast cancer risk. Progesterone, an ovarian steroid hormone that peaks during the luteal phase of the menstrual cycle, promotes the expansi

br Experimental Procedures br Author Contributions br Acknow

2018-10-20

Experimental Procedures Author Contributions Acknowledgments M. Ieda was supported by research grants from JST CREST, JSPS, Keio University Program for the Advancement of Next Generation Research Projects, Banyu Life Science, Senshin Medical Research Foundation, and Takeda Science Foundatio

br Results br Discussion Many small molecules including two

2018-10-20

Results Discussion Many small molecules, including two reported by our group (Lee et al., 2013b), have been suggested to be selectively toxic to PSCs. However, as described previously (Knoepfler, 2009), despite the high potency and efficacy of small molecules that target hPSC-specific genes (e

Taken together our findings suggest that

2018-10-20

Taken together, our findings suggest that in PD, the heterozygous GBA-N370S mutation leads to the misprocessing of GCase, ER stress upregulation, and autophagic/lysosomal dysfunction in dopaminergic neurons. We propose that this combination of disturbances impairs protein homeostasis in dopamine neu

Using both standard RNA seq

2018-10-20

Using both standard RNA-seq and RNA-seq lipoxygenase analysis, we identified UPF1-regulated transcripts that are good candidates to be direct NMD targets in hESCs. There was considerable overlap between these candidate NMD target transcripts expressed in hESCs with previously identified high-confid

Finally we tested whether the B cell differentiation defect

2018-10-20

Finally, we tested whether the B cell differentiation defect in the OsxCre;iDTR mutants could be rescued in vivo by intravenous administration of recombinant IL-7 and IGF-1. Both controls and mutants were subjected to daily injections of DT, DT + IL-7, or DT + IGF-1 for 12 days. At the end of the re

Remarkably the changes described suggest

2018-10-20

Remarkably, the changes described suggest that in vivo reprogramming-induced dedifferentiation renders a change at telomeres toward a more “juvenile” state, which in turn resembles that of ESCs and adult stem purchase GW788388 (see Figure 7). In this regard, we have previously described telomerase-

Hello world!

2018-07-29