• In summary we have shown that

  2018-10-22

  

  In summary we have shown that various Matrigel coated microcarriers can support long term propagation of undifferentiated hESC. HES-2 and HES-3 were propagated for over 17 passages on Matrigel coated DE53 and Cytodex 1 microcarriers (over 11 passages). Similar results were reported by Nie et al. (20

• However when we started this work the

  2018-10-22

  

  However, when we started this work, the data from the zebrafish and mouse studies had just been released, and a strategy to induce hematopoiesis in a stem cell population that could be obtained in very large numbers in the autologous setting seemed feasible, and with potentially huge implications in

• Of the small number of genes

  2018-10-22

  

  Of the small number of genes whose transcription differed by ≥3-fold between the two treatment groups (39, 51, and 93 out of 48,701 probe sets for E+G−, E+G+, and E−G+ fractions) (Figure 3G), most were upregulated in the WNT3A/BMP4-treated EBs (37, 43, and 61 probe sets, respectively). In the GFP+ f

• br Results and Discussion In order to

  2018-10-22

  

  Results and Discussion In order to identify regulators of ISC behavior, we carried out a screen using esgGal4 in the posterior midgut. From this screen, Dbr was identified as a candidate. Single esg+ cells are interspersed in young flies (Figure 1A). In aged flies, esg+ cells are clustered, enlar

• KN-93 br Conflict of interest statement The following are th

  2018-10-22

  

  Conflict of interest statement The following are the supplementary data related to this article. Acknowledgments This research was supported by funds to R.C. and F.C. from the Italian Ministry of Education, University and Research (MIUR). Introduction Besides hematopoietic stem KN-93 (HS

• Due to the limited proliferative capability of CMs large

  2018-10-20

  

  Due to the limited proliferative capability of CMs, large scale production of CMs is based on the expansion of pluripotent hESC followed by a CM differentiation step (Chen et al., 2014). Scaling up of hESC expansion in monolayer (MNL) culture platform is problematic mainly due to surface area limita

• br Materials and methods br Results br

  2018-10-20

  

  Materials and methods Results Discussion Human olfactory stem cells can be grown in neurospheres (Reynolds and Weiss, 1992). Murrell et al. obtained human mucosa by biopsy and generated OSs. They separated olfactory mucosa into lamina propria and epithelium. After separately dissociated, ce

• br Material and methods br Results br Discussion In our

  2018-10-20

  

  Material and methods Results Discussion In our previous studies, we showed that SVCT2 plays an important role in BMSC differentiation, cell attachment, and migration (Fulzele et al, 2013; Sangani et al, 2014). We also demonstrated that the SVCT2 transporter is down-regulated by oxidative st

• br Conclusions br Acknowledgements br Background Much of the

  2018-10-20

  

  Conclusions: Acknowledgements Background Much of the recent attention paid to human bone marrow stromal mol to ml (hBMSCs) is a consequence of their promise in cell-based therapy (Yi and Song, 2012). Features of BMSCs claimed that beneficial may include the capacity to home to sites of dam

• DNA fingerprinting confirmed the genetic relation

  2018-10-20

  

  DNA fingerprinting confirmed the genetic relation of the derivative line to the parental somatic line (Fig. 1B). The latrunculin manufacturer exhibited normal karyotype (46, XY) upon G-band analysis (Fig. 1C). Pluripotency was verified by gene expression of pluripotent stem cell markers Oct4, Sox2

• We recently reported that iPSCs can

  2018-10-20

  

  We recently reported that iPSCs can be generated using non-modified mRNA (Arnold et al., 2012). In this report we will focus on the crucial steps for successfully applying this method. Material and methods Results Discussion Acknowledgments The work was supported by funding from the Ger

• CBP is implicated in different biological functions

  2018-10-20

  

  CBP is implicated in different biological functions with an essential role in maintaining normal germ cells development. CBP is expressed in primordial germ cells (PGCs) and in ESCs but is highly expressed in PGCs compared with ESCs. Proper levels of CBP have been shown to be important for hematopoi

• Dental pulp stem cells DPSCs also known as dental

  2018-10-20

  

  Dental pulp stem EZ Cap Reagent GG Supplier (DPSCs), also known as dental pulp-derived mesenchymal stem cells, are a multipotent adult stem cell population that has a high proliferative potential. DPSCs in this study isolated from adult teeth are easily accessible and cryopreservable for long perio

• Muscle resident Sca CD cells

  2018-10-20

  

  Muscle-resident Sca-1+CD45− flunixin meglumine were minimally responsive to HFD and/or endurance exercise. A significant reduction in VEGF gene expression was observed in MDSCs in response to HFD, yet no other changes were noted in MDSC relative quantity or the expression of genes related to ECM re

• br Introduction A key question

  2018-10-20

  

  Introduction A key question in cardiovascular biology is to what degree the heart is able to regenerate after tissue damage from either cardiac stem cells or cardiomyocyte division. Cardiovascular disease including myocardial infarct is currently one of the leading causes of death worldwide, and

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